Transmission electron microscope Titan Krios (Krios)

Guarantor:
Michal Babiak
Instrument status:
Operational, 19.11.2020 10:30
Equipment placement:
Masarykova univerzita, CEITEC UKB
Research group:
CF: Cryo-Electron Microscopy and Tomography Core Facility
Detailed description:
Transmission electron microscope (TEM) operating at 80-300 kV that is equipped with a direct detector (Falcon II). This microscope allows automated data acquisition of purified proteins and their complexes to the highest possible resolutions (~3A} nowadays. Additionally, the microscope is also equipped with a Gatan energy filter with a 4k CCD camera, which allows elimination of inelastically scattered electrons. The microscope is therefore suitable for cryo-electron tomography of pleiomorfic protein complexes and cellular compartments. Data collection is fully automated by programs EPU (single particle analysis) and Tomo (tilt series).