PhD topics

Introducing and optimizing CRISPR/Cas methodology in plants.

The main task of the project will be implementing and optimizing CRISPR/Cas methodology in the laboratory. Set of suitable vectors will be designed by cloning methodology (Gibson assembly, Gateway etc.). Plant mutant lines will be generated firstly in Arabidopsis then in crops like Brassica, wheat, or barley. Genes of interest will be chosen mainly from cytokinin biosynthesis or signalling families, e.g. log genes. Regeneration and cultivation protocols will be established to obtain CRISPR/Cas lines. These protocols are expected to become part of the regular offer of services that are available from the Plant Sciences Core Facility. Results will be published in impacted journals.

Supervisor:  Mgr. Markéta Pernisová, Ph.D.